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OJB©
Online
Journal of Bioinformatics©
Onl J Bioinform©
Established 1995
ISSN 1443-2250
Volume 26 (1) :10-21, 2025.
Untranslated lactoferrin
gene in cows with single nucleotide polymorphism.
A Hajibemani,
H Sharifiyazdi, A Mirzaei, A Rowshan Ghasrodashti.
1Department of Clinical Science, School of Veterinary Medicine, Shiraz
University, Shiraz 71345, Iran; 2Islamic Azad University, Kazerun
Branch, Kazerun, Iran
ABSTRACT
Hajibemani A, Sharifiyazdi
H, Mirzaei A, Ghasrodashti
AR., Untranslated lactoferrin
gene in cows with single nucleotide polymorphis, Onl J Bioinform., 26 (1): 10-21, 2025.
Lactoferrin (Lf) antimicrobial protein is found in
uterine discharges of cows and allelic diversity associated with susceptibility to uterine
infections. We report single nucleotide polymorphism genes in
5' untranslated region (5'-UTR, position +32) of lactoferrin
gene by allele-specific PCR in cows with and without uterine infections. Blood was
taken from 89 multiparous Holstein dairy cows with and 38 controls
without history of uterine infections. Results
suggested presence of polymorphisms (G > C) in 5'-UTR in all cows and
allele specific PCR was 100% similar to sequencing. We found no
correlation between disease and SNP in 5'-UTR. However
we did find difference in mean reproductive parameters
of cows without polymorphism compared with SNP in 5'-UTR.
Cows with +32:CC genotype and +32:GC genotype SNP in
UTR had lower average of services per conception and days open compared with
+32:GG genotypes (P < 0.05). However, no significant difference in the
calving to first service was found between these genotypes (p > 0.05).
Keywords: Lactoferrin; polymorphism; uterine infection; cow.