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OJBTM
Online Journal of
Bioinformatics ©
Volume
11 (2): 317-326, 2010
Molecular identification of Pinctada
oysters by mitochondrial DNA analysis
Yassien MH1,
Obata M2, Iman MK2 and Komaru
A3
1National
Institute of Oceanography and Fisheries and 2National
Research Center, Egypt.3Faculty
of Bioresources, Mie
University, Tsu City, Mie
Prefecture, Japan.
ABSTRACT
Yassien MH, Obata M, Abumourad
MK, Komaru A., Molecular identification of Pinctada oysters by mitochondrial DNA analysis, Online J
Bioinformatics, 11 (2): 317-326, 2010 P. fucata and radiata
pearl oysters were differentiated through amplification of partial 16SrRNA and
CO1 gene using primer sets. CO1 gene had the same nucleotide count (nt) for P. fucata and radiata (684 bp) with 660
bps and 2 open reading frames encoding a protein of 220 aa in P. fucata and 654 bp three open
reading frames encoding a protein of 218 aa in radiata. P. fucata and
P. radiata had 88.3 and 91.5% similarity,
respectively, enabling this distinction. DNA analysis and PCR suggest
that pinctada species are genetically different
but belong to a same ancestor.
KEY WORDS: Cytochrome C oxidase subunit 1,
16SrRNA, Pinctada fucata,
Pinctada radiata,
polymerase chain reaction, Molecular identification.
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