©2025-2036
All Rights Reserved. Online Journal of Bioinformatics.
You may not store these pages in any form except for your own personal use. All
other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the
before mentioned must be gained in writing from the publisher. This
article is exclusively copyrighted in its entirety to OJB publications. This
article may be copied once but may not be reproduced or re-transmitted without
the express permission of the editors.
OJB©
Online Journal of Bioinformatics©
Onl J Bioinform©
Established 1995
ISSN 1443-2250
Volume 26 (2) : 70-76, 2025
Intron-2 variability of leptin
gene in buffalo.
Srikala R, Ravikumar G, Narang
R, Satyendra PS, Amrita C, Rajni
G.
Molecular
Cytogenetics Lab, Department of Animal Breeding and Genetics,
Ludhiana, India.
ABSTRACT
A study on
leptin gene was conducted to detect the variability at nucleotide level in reverine buffaloes. The PCR-RFLP of 423 bp
amplicon of leptin gene with Hph I, Sau 3A I
and Alu I
revealed monomorphism at these loci in Mehsana, Surti and Nili-Ravi breeds of
buffaloes. However on sequencing the amplicon in these breeds variability at
nucleotide positions 43, 47, 51, 70, 71, 76, 89, 94, 99, 150, 194, 213, 267,
315, 335,411 and 415 was identified. The comparison between buffalo sequences
and other species revealed changes of nucleotides at several positions. The
sequences were submitted in the NCBI gene bank with accession numbers:
DQ497641, DQ831142 and DQ831143. On phylogenetic analysis of nucleic acid
sequences all the buffalo leptin sequences clustered into a monophyletic group.
The bovids clustered clearly from Capra hircus
and Ovis aries
cluster.
Key words : Sequence,
polymorphism, Bubalus bubalis,
Leptin.
FULL-TEXT (SUBSCRIBE OR PURCHASE TITLE)