©2025-2036
All Rights Reserved. Online Journal of Bioinformatics.
You may not store these pages in any form except for your own personal use. All
other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the
before mentioned must be gained in writing from the publisher. This
article is exclusively copyrighted in its entirety to OJB publications. This
article may be copied once but may not be reproduced or re-transmitted without
the express permission of the editors.
OJB©
Online
Journal of Bioinformatics©
Onl
J Bioinform©
Established
1995
ISSN 1443-2250
Volume
26 (1) :32-36, 2025
Epitopes M2e-HA2 clone, expression and purification of
Influenza A virus in Escherichia coli
Masoud Moghadaszadeh, Mehdi Golchin,
Hadi Tavakkoli, Reza Ghanbarpour
12Department(s) of
Pathobiology, 3Clinical Sciences, Shahid Bahonar University of Kerman, Iran.
ABSTRACT
Moghadaszadeh
M, Golchin M, Tavakkoli H, Ghanbarpour
R.,
Epitopes M2e-HA2 clone, expression and
purification of Influenza A virus in Escherichia coli,, Onl J Bioinform., 26 (1): 32-36, 2025. Influenza can
be controlled by vaccination but the virus due to its high mutation rate, has
limited efficacy. Influenza viruses possess conserved epitopes matrix-2 protein
ecto-domain (M2e) and HA2 that could be used in a vaccine.
In this work, M2e-HA2 peptide was expressed in E. coli and then purified.
The M2e-HA2 gene from influenza A virus was then synthesized
and cloned into pGS21vector. The construct was transferred into E. coli BL21
(DE3) and induced using IPTG. Expression of recombinant peptide was confirmed
by western blot assay using anti-GST monoclonal antibody. The expressed peptide-GST
was purified from bacterial lysate by IMAC chromatography. The resulting
sequence revealed that the M2e-HA2 gene was cloned in the vector. SDS-PAGE
electrophoresis of purified peptide demonstrated a strong single band. Western
blot analysis showed a single band in correct position. The purified peptide could
be tested In vivo against influenza
virus infection.
Key words: Cloning,
Expression, Purification, M2e-HA2, Escherichia coli, Influenza vaccine
FULL-TEXT (SUBSCRIBE OR PURCHASE TITLE)