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OJB©

Online Journal of Bioinformatics©

Onl J Bioinform©

Established 1995

ISSN  1443-2250

Volume 27 (2) : 61-98, 2026.

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Conservation of acetoacetyl-CoA reductase in bacteria

Feroz Khan, Satarudra Prakash Singh, BN Mishra.

Department of Biotechnology, Institute of Engineering & Technology, UP Technical University, Lucknow, India.

ABSTRACT

Feroz Khan, Satarudra Prakash Singh, BN Mishra., Conservation of acetoacetyl-CoA reductase in bacteria, Onl J Bioinform., 27 (2) : 61-98, 2026. For evaluation of PFM performance, predicted results were compared with PhoB matrix of RegulonDB; an E. coli regulatory network database. PhoB activator binding sites were identified in phbB gene orthologs of poly-β-hydroxybutyrate (PHB) metabolic pathway. We found PhoB binding sites conserved in bacterial genomes which had PHB biosynthetic metabolic pathway genes and those encoding orthologous PhoB transcription factor. We find that for efficient PHB biosynthesis, acetoacetyl-CoA reductase is a limiting enzyme and under phosphate limitation, PHB biosynthesis pathway would be induced optimally.

Key-words:- PHA biopolymer, phbBCA operon, alternative regulation in PHA biosynthesis metabolic pathways, phbB gene orthologs, pho-box in PHB biosynthetic pathway, PhoB transcription factor, E. coli & Acinetobacter sp..