©2021-2032 All Rights Reserved. Online Journal of Bioinformatics. You may not store these pages in any form except
for your own personal use. All other usage or distribution is illegal under
international copyright treaties. Permission to use any of these pages in any
other way besides the
before mentioned must be gained in writing from the publisher. This article is
exclusively copyrighted in its entirety to onlinejournals@gmail.com
publications. This article may be copied once but may not be, reproduced or re-transmitted
without the express permission of the editors. Linking: To link to this page or
any pages linking to this page you must link directly to this page only here
rather than put up your own page.
OJBTM
Online Journal of Bioinformatics©
Established 1995
ISSN 1443-2250
Volume 23 (3):258-267, 2022.
Identification
and sequence analysis of bovine toll like receptor gene.
Animal Genomics Lab, Department of Animal Biotechnology, GADVASU,
Ludhiana, Punjab, India
ABSTRACT
Shah SM, Kumar
R, Brah GS., Identification and sequence analysis of bovine
toll like receptor gene, OnlJ Bioinform.,
23 (3):258-267, 2022. Toll-like
receptor 2 (TLR2) is the most promiscuous TLR implicated in signaling induced
by Gram-positive cell walls, peptidoglycan and mycobacterial factors, GPI
anchors (Trypanosoma cruzi),
lipoarabinomann (Mycobacterium
tuberculosis), porins (Nesseria meningitides) and zymosan yeast cell
wall component and a number of endogenous ligands viz., necrotic cells and
their protein byproducts and heat shock proteins (HSP60, HSP 70 and GP96). We characterized
TLR2 gene in Murrah buffalo (Bubalus bubalis) by amplifying
and cloning a 1152bp sequence submitted
to Genbank with accession number GU441859. The
sequence shared 98% identity with Capra hircus, 97% Bos taurus, Bos indicus and Bison bison, 96% Boselaphus tragocamelus, 94% Ovis aries, 82% Sus scrofa and Eqqus cabalus and 81% with Canis lupus familaris. The sequence had 95% similarity
with Bison bison,
94% Bos indicus and Bos taurus,
93% Boselaphus tragocamelus,
90% Ovis aries, 89% Capra hircus,
73% Sus scrofa, 71% Equus caballus and Canis lupus familaris
and 44% with Gallus gallus.
Phylogenetic analysis that buffalo clustered with other bovids and Capra hircus, but diverged from Equus cabalus, Sus scrofa and Gallus gallus. Analysis of synonymous and nonsynonymous
substitutions per site suggested that nucleotide sequences coding for the TLR2
gene were not under positive selection and null hypothesis of strict-neutrality
was deduced.
Key words: Cloning,
Characterization, Toll like receptor, Buffalo, Phylogeny.