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Online Journal of
Bioinformatics©
Established 1995
ISSN 1443-2250
Volume
19(3):258-270, 2018.
Extraction of
protein surface information from its aggregate.
Hrishikesh Mishra, Tapobrata
Lahiri.
Division of
Applied Sciences and Indo-Russian Center for Biotechnology, Indian Institute of
Information Technology, Allahabad, India.
ABSTRACT
Mishra H, Lahiri T.,
Extraction of protein surface information from its aggregate, Onl J Bioinform., 19(3):258-270, 2018. Recent studies show that protein
surface roughness profile could be used to identify small molecule attachment
sites. Moreover, rough protein assembly carries a unique signature of its seed
protein. Using these 2 charafcteristics we mapped a
protocol for roughness profile of a protein from intensity level based multi
fractal dimension (ILMFD) of its heat-denatured aggregates. The steps used were
extraction of feature, ILMFD from microscopic images of heat denatured protein
aggregate (HDPA), followed by mapping of ILMFD into Surface Roughness Index
(SRI) through suitably chosen artificial neural network architecture. Our
approach may determine a protein’s surface property without depending on its
structure.
Key Words: Protein surface roughness, Intensity level based multifractal dimension, Heat denatured protein aggregate, Artificial Neural Network
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